Validating internal controls for quantitative plant gene expression studies

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Therefore, we hypothesize that ACS gene might play an important role in poplar plants in mitigating stresses resulting from pollutant exposure.The homolog of poplar ACC synthase gene was PCR amplified from Arabidopsis thaliana and cloned into p BINmgfp5-er vector, featuring a constitutive cauliflower mosaic virus promoter removing the native jellyfish green fluorescent (GFP) gene. Poster #: 1 Campus: CSU Northridge Poster Category: Agriculture/Biofuels/Environment Keywords: Arabidopsis thaliana, Genetic engineering, Weather pollution Project Title: Overexpression of an aminocyclopropane-1-carboxylic acid synthase gene in Arabidopsis thaliana and its effect on removing environmental pollutants Author List: Ramadoss, Niveditha; Graduate, Biology, California State University, Northridge, Presenting Author Gupta, Dinesh; Washington University, St.Louis Guenther, Alex; University of California, Irvine Basu, Chhandak; Biology, California State University, Northridge Abstract: Rise in air pollution has led to increasing cancer, several respiratory and cardiac illnesses in humans.Please submit a cover letter, CV, and contact information for references to Dr. This will require field observations and experimentation, laboratory measures, and the synthesis and analysis of large, complex ecological datasets.Anna Schoettle ([email protected] at USFS Rocky Mountain Research Station) and Dr. This position will work closely with the BLM’s Colorado Plateau Native Plant Program’s Lead and in consistent consultation with the program’s geneticist.This cookie stores just a session ID; no other information is captured.Accepting the NEJM cookie is necessary to use the website.

Cells in all organisms regulate gene expression by turnover of gene transcripts (single stranded RNA): The amount of an expressed gene in a cell can be measured by the number of copies of an RNA transcript of that gene present in a sample.In order to robustly detect and quantify gene expression from small amounts of RNA, amplification of the gene transcript is necessary.The polymerase chain reaction (PCR) is a common method for amplifying DNA; for RNA-based PCR the RNA sample is first reverse-transcribed to complementary DNA (c DNA) with reverse transcriptase.This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed.For attribution, the original author(s), title, publication source (Peer J) and either DOI or URL of the article must be cited.

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